Shigetoshi Eda
Associate Professor
Shigetoshi Eda is Associate Professor in the Center for Wildlife Health (Dept. FWF). He received Ph.D. degree from Tokyo University of Pharmacy and Life Science (Japan). After working as a postdoctoral researcher at the University of California Riverside, he joined the university in 2003.
He is studying immunological reactions of cattle against surface molecules of Mycobacterium avium subsp. paratuberculosis (MAP), the causative agent of Johne's disease in livestock and wild animals. With Dr. Speer, Cathy Scott and graduate students, he developed a highly accurate diagnostic test for the disease and the research on Johne's disease diagnosis resulted in several scientific publications, articles on Angus Journal and Hoard's Dairyman Magazine, two patents, and six extramural grants & contracts. The diagnostic test was licensed to a veterinary diagnostic company and is planned to be commercialized in a couple of years. Their recent findings demonstrated that the technology used in the test was applicable to other infectious diseases in animals (e.g. bovine tuberculosis) and, therefore, the research has a potential in contributing to improvement of wildlife health issues. Since many animal infectious diseases are know to be zoonotic -- communicable to humans, the control of animal infectious diseases is also important for protecting people.
Based on the technology, he, with scientists at ORNL, is currently developing 'next-generation' diagnostic tests for animal and human infectious diseases through multilateral (Nanotechnology, Computer Science, Advanced Mathematical Techniques, and Proteomics/Genomics) collaborations with scientists in other departments on campus, other universities, and US Department of Agriculture.
He contributes to courses in the Wildlife Health curriculum, including co-teaching WFS 101 since 2005 and served as a member of thesis committee of three graduate students. Also, he leads the Kendo class in the Martial Art Club at UT.
Funding
"Optimization and Evaluation of Novel Absorbed Serum and Milk Enzyme-linked Immunosorbent Assays for Johne's Disease." (PI, USDA/NRI/CSREES) September, 2007
"Evaluation of Novel Absorbed Serum Enzyme-linked Immunosorbent Assays for Johne's Disease. Phase I" (PI, Veterinary diagnostic company)
"Development and Commercialization of a Novel Diagnostic Test for Staphylococcus aureus Infections in Humans and Animals" (PI, The 2008 University of Tennessee Research Foundation's Maturation Funding Program)
"Evaluation of Novel Absorbed Serum Enzyme-linked Immunosorbent Assays for Johne's Disease. Phase II" (PI, Veterinary diagnostic company)
"Development of an on-site diagnostic device for Johne's disease" (PI, Johne's Disease Integrated Program)
"Development of a hand-held diagnostic test for bovine tuberculosis" (PI, Animal Health Board, New Zealand)
Publications on current projects
1. S. Eda, B. Elliott, M. C. Scott, W. R. Waters, J. Bannantine, R. Whitlock, and C. A. Speer New Method of Serological Testing for Mycobacterium avium subsp. paratuberculosis (Johne's Disease) by Flow Cytometry. Foodborne Pathogens and Diseases (2005) 2 (3) 250-62.
2. C. A. Speer, M. C. Scott, J. Bannantine, W. R. Waters, Y. Mori, R. Whitlock, and S. Eda 2006. A novel ELISA for the diagnosis of Mycobacterium avium subsp. paratuberculosis infections (Johne's disease) in cattle. Clinical and Vaccine Immunology 13(5): 535-540.
3. S. Eda, J. P. Bannantine, W. R. Waters, Y. Mori, R. H. Whitlock, M. C. Scott and C. A. Speer. 2006. A Highly Sensitive and Subspecies-Specific Surface Antigen Enzyme-Linked Immunosorbent Assay for the Diagnosis of Johne's Disease Clinical and Vaccine Immunology 13(8): 837-844
Publicaitons on human malaria
1. S. Eda, J. Lawler, and I. W. Sherman 1999. Plasmodium falciparum-infected erythrocyte adhesion to the type 3 repeat domain of thrombospondin-1 is mediated by a modified band 3 protein. Molecular and Biochemical Parasitology 100(2): 195-205
2. S. Eda, K. Eda, J. Prudhomme, and I. W. Sherman 1999. Inhibitory activity of human lactoferrin and its peptide on chondroitin sulfate A-, CD36- and thrombospondin-mediated cytoadherence of Plasmodium falciparum-infected erythrocytes. Blood 94(1): 326-332
3. S. Eda, E. Winograd, S. Eda, Irwin W. Sherman 2004. Chemical modifications of band 3 protein affect the adhesion of Plasmodium falciparum-infected erythrocytes to CD36. Molecular and Biochemical Parasitology 136(2): 243-8.
Publicaitons on apoptosis
1. S. Eda, M. Yamanaka, and M. Beppu 2004. Carbohydrate-mediated phagocytic recognition of early apoptotic cells undergoing transient capping of CD43 glycoprotein. Journal of Biological Chemistry 279 (7): 5967-5974.
2. M. Yamanaka, S. Eda, and M. Beppu. 2005. Carbohydrate chains and phosphatidylserine successively work as signals for apoptotic cell removal. Biochemical and Biophysical Research Communications. 328: 273-280
3. K. Hirano, S. Eda, Y. Miki, A. Hayashi, M. Yamanaka, T. Ito, Y, Hirai, R. Sato, and M. Beppu. 2005. A multifunctional shuttle protein nucleolin is a macrophage receptor for apoptotic cells. Journal of Biological Chemistry 280(47): 39284-93.
All publications
Computer skills
A computer programming language, Perl, has been used extensively in Bioinformatics. Using the language, he wrote many computer programs for his research. One of the programs is for organizing scientific PDF files and has been downloaded by, at least, 500 people (more information). Also, he wrote computer programs for amino acid sequence alignment, phylogenic tree analysis, biostatistics, machine learning methods/artificial intelligence, and mathematical modeling of infectious diseases (available online). He is also familiar with other programming languages, such as Java and C++. Using (D,X)HTML, CSS, XML, XSLT, Ajax, and Perl/CGI, he created the official website of the Plant Biotechnology Building and Genomics hub at the University of Tennessee Knoxville and have served as a webmaster of the page for four years.
Technical background
He has used four different flow cytometers and their softwares in the past 14 years. Also, he has experience in using epifluorescent microscopes, confocal microscope, scanning electron microscopes, amino acid sequencer, and thermocycler, in addition to basic research instruments. High-throughput screening works have been carried out using phage display libraries and combinatorial random peptide library. He prepared monoclonal as well as polyclonal antibodies using mice and rabbits, respectively. He has knowledge and experience in purification of proteins using various chromatographic techniques and in analysis of the purified proteins using immunological methods, such as Western blotting, ELISA, cell blotting etc. He also has experience in DNA manipulations, plasmid preparation, and DNA sequencing.
Contact Information
Shigetoshi Eda
366 Plant Biotechnology Bldg
(865) 974-5008
Email: seda@utk.edu
Current graduate student
Ashutosh Wadhwa, Ph.D. candidate