Hydrogeology Research Group Homepage

Faculty and Staff

Research Areas

Graduate Students

Graduate Student Opportunities

Links

MELISSA E. LENCZEWSKI


University of Tennessee
Department of Geological Sciences
306 G & G Building
Knoxville, TN 37996-1410
(865) 974-2366
mlenczew@utk.edu

EDUCATION:

Ph.D. Candidate, Geological Sciences, University of Tennessee, Knoxville
1997-Present
Advisor: Dr. Larry D. McKay
Dissertation: Biodegradation of Trichloroethylene in Fractured Shale Saprolite
Graduation: Summer/Fall 2000 (expected)

Master of Science, Soil, Water and Environmental Science, University of Arizona, Tucson
1991-1993
Advisor: Dr. Charles P. Gerba
Thesis: Comparative Transport of Bacteriophage and Microspheres in an Aquifer under Forced-Gradient Conditions

Bachelor of Science, Microbiology, minor in Chemistry, University of Arizona, Tucson
1987-1991

NON-ACADEMIC RESEARCH EMPLOYMENT:

1993-1997 Amway Corp. Analytical Services, Ada, MI, Research Scientist, Microbiology

RESEARCH EXPERIENCE:


DISSERTATION RESEARCH:

University of Tennessee
  • Research combines techniques from hydrogeology, microbiology, and molecular biology to address the issue of biodegradation of trichloroethylene (TCE) in fractured clay-rich materials.
  • Evaluation of natural attenuation of TCE in fractured and weathered shale bedrock at a former disposal site at Oak Ridge Reservation, Oak Ridge, TN. The research includes both field-scale evaluation of natural attenuation of TCE in an existing 50 m long plume and laboratory scale experiments to investigate geochemical and microbial controls on TCE degradation in these materials.
  • The investigation includes long-term injections of dissolved phase TCE into two samples (anaerobic and aerobic) of undisturbed fractured saprolite from uncontaminated sites at the Oak Ridge Reservation. During the experiment, the effluent from the columns are monitored for TCE, daughter products, inorganic chemicals, and assayed to examine microbial communities by traditional microbiology and molecular techniques.

PREVIOUS RESEARCH EXPERIENCE:

Amway Corporation
  • Research experience includes preservative efficacy testing and sterility testing for cosmetics and cleaning products with emphasis on new methods and method improvements.
  • Developed a minimum inhibitory concentration (MIC) based method to determine the optimal concentration of preservatives in personal and cleaning products. The research resulted in two publications in a peer-reviewed journal (Lenczewski et al., 1996; and Lenczewski and Kananen, 1998).
  • Lead scientist in potable water testing for heterotrophic plate count bacteria and total coliform; also perform work related to water treatment systems.
University of Arizona: Graduate Research
  • Graduate research focused on experimental investigation of the factors influencing transport of colloids in granular aquifer material. The investigations included a field experiment studying the effects of pH and heterogeneities on the transport of bacteriophage and microspheres at the Borden site, Canada. The research was subsequently published in a peer-reviewed journal (Bales et al., 1997).
Geological Survey of Denmark and Greenland
  • Research focused on tracer experiments in fractured porous media. This included investigations in a laboratory soil column and field tracer experiments in fractured till overlying a chalk aquifer in Denmark and led to publication of two peer-reviewed journal papers (Hinsby et al., 1996; McKay et al., in press). My participation began while I was an MS student and continued during summer vacations while I was employed at Amway. I was also involved in setting up a monitoring program to monitoring pathogenic microorganisms in Danish groundwater.
University of Arizona: Undergraduate
  • Worked in a certified laboratory under the direction of Dr. Charles Gerba testing for the presence of enteric viruses, Giardia, and Cryptosporidium in water using tissue culture and immunofluorescence techniques.

REFEREED PUBLICATIONS:

L. McKay, J. Fredericia, M. Lenczewski, J. Morthorst, and K. E. S. Klint. 1999. Spatial Variability of Contaminant Transport in a Fractured Till, Avedore Denmark. Nordic Hydrology. In Press.

M. Lenczewski and L. Kananen. 1998. Automated to Optimize Antimicrobial Preservative Levels in Personal and Home Care Products. Journal of AOAC International. 81:534-539.

R. C. Bales, S. Li, T.C.J. Yeh, M. E. Lenczewski, and C. P. Gerba. 1997. Bacteriophage and Microsphere Transport in Saturated Porous Media: Forced-Gradient Experiment at Borden, Ontario. Water Resources Research. 33: 639-648.

K. Hinsby, L. D. McKay, P. Jorgensen, M. Lenczewski, and C. Gerba. 1996.Fracture Aperture Values and Contaminant Migration in a Column of Clay Till. Ground Water. 34:1065-1075.

M. Lenczewski, S. T. McGavin, and K. J. VanDyke. 1996. Comparison of Automated and Traditional Minimum Inhibitory Concentration Procedures for Microbiological Cosmetic Preservatives. Journal AOAC International. 79:1294-1299.

MANUSCRIPTS IN PREPARATION:

M. Lenczewski, P. Jardine, L. McKay, D. Haun, and S. Pickrell. Natural Attenuation of Trichloroethylene in Fractured Shale Bedrock. In preparation for submission to Journal of Contaminant Hydrology.

M. Lenczewski, L. McKay, A. Layton and C. Knight. Methods for Determining Biodegradation of Trichloroethylene in Large Undisturbed Columns of Fractured Shale Saprolite. For submission to Bioremediation Journal.

M. Lenczewski, L. McKay, and A. Layton. Microbial Community Structure Shifts During Biodegradation of Trichloroethylene in Large Undisturbed Columns of Fractured Shale Saprolite. For submission to Applied and Environmental Microbiology.

M. Lenczewski, L. McKay, A. Layton, and P. Jardine. Comparison of Aerobic and Anaerobic Biodegradation of Trichloroethylene in Columns of Fractured Shale Saprolite. For submission to Water Resources Research.

SELECTED ABSTRACTS:

M. Lenczewski, L. McKay, and A. Layton. 1999. Trichloroethylene Biodegradation in Large Undistributed Columns of Fractured Weathered Shale in East Tennessee. International Symposium on Subsurface Microbiology. Vail, CO.

M. Lenczewski, L. McKay, and A. Layton. 1999. Biodegradation of Trichloroethylene in Fractured Weathered Shale in East Tennessee. American Society for Microbiology. Chicago, IL.

M. Lenczewski, L. McKay, and J. Sanseverino. 1999. Biodegradation of TCE in Fractured Shale Saprolite. Ninth Tennessee Water Resources Symposium. Nashville, TN.

M. Lenczewski, L. McKay, J. Sanseverino, and C. Knight. 1998. Sorption and Microbiological Factors Controlling the Fate and Transport of TCE in Fractured Shale Saprolite. Conference on Mass Transport in Fractured Aquifers and Aquitards, University of Copenhagen, Copenhagen, Denmark.

M. Lenczewski, L. Kananen, B. Hubbel, and A. Meszaros. 1997. Evaluation of ESP Automated Industrial Microbial Testing System for Cosmetic and Personal Care Products. American Society for Microbiology. Miami Beach, FL.

M. Lenczewski, P. Watt, J. Aamand, L. McKay, and J. Fredericia. 1996. Development of a Groundwater Monitoring Program in Denmark. American Society for Microbiology, New Orleans, LA.

B. R. Wilson, M Lenczewski, M. Pelka, J. Naranjo, and C. Gerba. 1995. Evaluation of the use of 1-3 µm Microspheres to Predict Cryptospordium oocysts and Giardia cyst Removal Efficiency During Water Treatment Filtration. American Society for Microbiology, Washington, DC.

M. Lenczewski, K. Hinsby, and L. McKay. 1994. Transport of PRD-1 and MS-2 Bacteriophage through a Fractured Danish Till. American Society for Microbiology, Las Vegas, Nevada.

S. Li, R. C. Bales, T-C. J. Yeh, M. E. Lenczewski, and C. P. Gerba. 1993. Forced Gradient Test of Virus Transport in Porous Media. American Geophysical Union, Baltimore, Maryland.

L. McKay, K. Hinsby, J. Morthorst, and M. Lenczewski. 1993. Field and Laboratory Tracer Experiments in Fractured Clayey Till in Denmark. Geological Society of America, 1993 Annual Meeting.

M. Lenczewski. 1993. Bacteriophage Transport through a Fractured Danish Till. Arizona Universities Student Environmental Conference. Flagstaff, Arizona.

M. Lenczewski, K. Hinsby, P. Jorgensen, and L. McKay. 1993. Transport of PRD-1 and MS-2 Bacteriophage Through a Fractured Danish Till. Arizona Branch American Society for Microbiology, Tucson, Arizona.

D. Moore, T-C. Yeh, R. Bales, C. Gerba, and M. Lenczewski. 1992. Transport of MS-2 and PRD-1 Viruses in a Three Dimensional Physical Aquifer Model. American Geophysical Union, San Francisco, California.

M. Lenczewski, S. Li, R. Bales, and C. Gerba. 1992. Comparative Transport of Bacteriophage and Microspheres in an Aquifer Under Forced-Gradient Conditions. Arizona Branch American Society for Microbiology, Flagstaff, Arizona.

TEACHING EXPERIENCE:


Introductory Physical Geology Lab and Environmental Geology Lab: Laboratory instruction; writing quizzes and exams

Hydrogeology: Grading homework and tutoring students

Field Methods in Hydrogeology: Field exercises at the UT field hydrology teaching site which includes assisting in design and supervision of drilling and coring operations plus installation of multi-level wells; setup and supervision of pump/slug tests; and site surveying.

Field Camp-Hydrogeology Section: Field exercises covered methods of water sampling and monitoring, water quality and microbiology; also aided in carrying out pump/slug tests and site surveying. Laboratory experiments included chloride tracer tests along with lectures on types of contamination, sources of groundwater contamination, health or environmental risks, and physical principles of contaminate transport.

CERTIFICATION AND HONORS:

1999 Waste Management Research and Education Institute Fellowship
1999 University of Tennessee, Department of Geological Science, Exceptional Professional Promise Award
1998 University of Tennessee, Department of Geological Science, Best student presentation.
1996 Registered Microbiologist. American Society for Microbiology.
1995 Amway Corp., Nomination for R&D Creativity Award.
1993 Arizona Universities Student Environment Conference, Best student paper presentation.
1992 American Society for Microbiology Travel Grant, Best graduate student presentation at the Arizona Branch annual meeting.

Email questions, comments or concerns to: mlenczew@utk.edu