DIDEOXY

Graphics Version: Text Version: Index to Other presentations

Please send comments to khughes@utk.edu

Slide 2 -Dideoxy nucleoside triphosphates

Slide 3 - Nested DNA fragments

Slide 4 - Primer binding

Slide 5 - DNA polymerase adds nucleotides

Slide 6 - The first DNA copy is extended

Slide 7 - Termination of fragment with a dideoxy nucleotide<-Termination of the first strand by incorporation of a dideoxy nucleoside triphosphate>

Slide 8 - Release of the first DNA copy

Slide 9 - A second strand is copied and terminated

Slide 10 - A second strand is released

Slide 11 - A third strand is copied

Slide 12 - A third strand is released

Slide 13 - The three copied strands are illustrated

Slide 14 - DNA mixtures are loaded on gels

Slide 16 - Glass plates used for DNA sequencing gels

Slide 17 - The top plate is lowered on the bottom plate

Slide 18 - The plates are taped together

Slide 19 -  Polyacrylamide is poured between the plates

Slide 20 - Plates are placed in the automated sequencer and buffer tanks are installed

Slide 21 - DNA sequencing reactions are carried out and prepared for loading on the gel

Slide 22 - - DNA samples are loaded in wells in the gel

Slide 24- Fluorescent bands on a gel after electrophoresis

Slide 25 - Printout of laser scan of gel

Slide 26 - End of presentation