Electron Energy Loss Spectroscopy
The fast electrons in a transmission electron microscope scatter inelastically at the sample and transfer energy to the atoms. The energy these electrons transfer (or loose) is specific to the elements in the sample, their coordination, bonding, optical and electronic properties.
To observe an electron energy loss spectrum as shown above you need an electron energy loss spectrometer on a TEM.
To analyse the chemical content of a sample, we use a new method called "QuantiTEM", and to extract bonding information of the atoms, we analyse the shape of the element specific ionization edges called electron near edge structure (ELNES) .