** CD-14 **

CD-14

Gene name:	CD14
Gene type:	Protein coding
Gene description:	CD14 antigen
Locus ID:	929
Gene Location:	5q31.1
Organism:	Homo sapiens
Lineage:	Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo
Summary:	CD14 is a surface protein preferentially expressed on monocytes/macrophages. It binds lipopolysaccharide binding protein and recently has been shown to bind apoptotic cells. CD14 is a single-copy gene encoding 2 protein forms: a 50- to 55-kD glycosylphosphatidylinositol-anchored membrane protein (mCD14) and a monocyte or liver-derived soluble serum protein (sCD14) that lacks the anchor. Both molecules are critical for lipopolysaccharide (LPS)-dependent signal transduction, and sCD14 confers LPS sensitivity to cells lacking mCD14. Increased sCD14 levels are associated with inflammatory infectious diseases and high mortality in gram-negative shock.
Link to the record entry:	Entrez Gene and OMIM
Total gene size:	1456 bp
mRNA length:	1368 bp
Protein length:	376 aa

mRNA		Protein
EXON		Coding EXON		INTRON
coords	length	coords	length	coords	length
1 - 123	123 bp	121 - 123	3 bp	124 - 211	88 bp
212 - 1456	1245 bp	212 - 1336	1125 bp
Total:	1368 bp	Total:	376 aa	Total:	88 bp

Sequence of:
First Exon	1 61 121	GGCAGCCGAA GAGTTCACAA GTGTGAAGCC TGGAAGCCGG CGGGTGCCGC TGTGTAGGAA AGAAGCTAAA GCACTTCCAG AGCCTGTCCG GAGCTCAGAG GTTCGGAAGA CTTATCGACC ATG
Second Exon	1 61 121 181 241 301 361 421 481 541 601 661 721 781 841 901 961 1021 1081 1141 1201	GAGCGCGCGT CCTGCTTGTT GCTGCTGCTG CTGCCGCTGG TGCACGTCTC TGCGACCACG CCAGAACCTT GTGAGCTGGA CGATGAAGAT TTCCGCTGCG TCTGCAACTT CTCCGAACCT CAGCCCGACT GGTCCGAAGC CTTCCAGTGT GTGTCTGCAG TAGAGGTGGA GATCCATGCC GGCGGTCTCA ACCTAGAGCC GTTTCTAAAG CGCGTCGATG CGGACGCCGA CCCGCGGCAG TATGCTGACA CGGTCAAGGC TCTCCGCGTG CGGCGGCTCA CAGTGGGAGC CGCACAGGTT CCTGCTCAGC TACTGGTAGG CGCCCTGCGT GTGCTAGCGT ACTCCCGCCT CAAGGAACTG ACGCTCGAGG ACCTAAAGAT AACCGGCACC ATGCCTCCGC TGCCTCTGGA AGCCACAGGA CTTGCACTTT CCAGCTTGCG CCTACGCAAC GTGTCGTGGG CGACAGGGCG TTCTTGGCTC GCCGAGCTGC AGCAGTGGCT CAAGCCAGGC CTCAAGGTAC TGAGCATTGC CCAAGCACAC TCGCCTGCCT TTTCCTGCGA ACAGGTTCGC GCCTTCCCGG CCCTTACCAG CCTAGACCTG TCTGACAATC CTGGACTGGG CGAACGCGGA CTGATGGCGG CTCTCTGTCC CCACAAGTTC CCGGCCATCC AGAATCTAGC GCTGCGCAAC ACAGGAATGG AGACGCCCAC AGGCGTGTGC GCCGCACTGG CGGCGGCAGG TGTGCAGCCC CACAGCCTAG ACCTCAGCCA CAACTCGCTG CGCGCCACCG TAAACCCTAG CGCTCCGAGA TGCATGTGGT CCAGCGCCCT GAACTCCCTC AATCTGTCGT TCGCTGGGCT GGAACAGGTG CCTAAAGGAC TGCCAGCCAA GCTCAGAGTG CTCGATCTCA GCTGCAACAG ACTGAACAGG GCGCCGCAGC CTGACGAGCT GCCCGAGGTG GATAACCTGA CACTGGACGG GAATCCCTTC CTGGTCCCTG GAACTGCCCT CCCCCACGAG GGCTCAATGA ACTCCGGCGT GGTCCCAGCC TGTGCACGTT CGACCCTGTC GGTGGGGGTG TCGGGAACCC TGGTGCTGCT CCAAGGGGCC CGGGGCTTTG CCTAAGATCC AAGACAGAAT AATGAATGGA CTCAAACTGC CTTGGCTTCA GGGGAGTCCC GTCAGGACGT TGAGGACTTT TCGACCAATT CAACCCTTTG CCCCACCTTT ATTAAAATCT TAAAC
mRNA	1 61 121 181 241 301 361 421 481 541 601 661 721 781 841 901 961 1021 1081 1141 1201 1261 1321	ccggccggcc gaagagttca caagtgtgaa gcctgaagcc gccgggtgcc gctgtgtaga aagaagctaa agcacttcca gagcctgctg agctcagagg ttcggaagac ttatcgacca tggagcgcgc gtcctgcttg ttgctgctgc tgctgccgct ggtgcacgtc tctgcgacca cgccagaacc ttgtgagctg gacgatgaag atttccgctg cgtctgcaac ttctccgaac ctcagcccga ctggtccgaa gccttccagt gtgtgtctgc agtagaggtg gagatccatg ccggcggtct caacctagag ccgtttctaa agcgcgtcga tgcggacgcc gacccgcggc agtatgctga cacggtcaag gctctccgcg tgcggcggct cacagtggga gccgcacagg ttcctgctca gctactggta ggcgccctgc gtgtgctagc gtactcccgc ctcaaggaac tgacgctcga ggacctaaag ataaccggca ccatgcctcc gctgcctctg gaagccacag gacttgcact ttccagcttg cgcctacgca acgtgtcgtg ggcgacaggg cgttcttggc tcgccgagct gcagcagtgg ctcaagccag gcctcaaggt actgagcatt gcccaagcac actcgcctgc cttttcctgc gaacaggttc gcgccttccc ggcccttacc agcctagacc tgtctgacaa tcctggactg ggcgaacgcg gactgatggc ggctctctgt ccccacaagt tcccggccat ccagaatcta gcgctgcgca acacaggaat ggagacgccc acaggcgtgt gcgccgcact ggcggcggca ggtgtgcagc cccacagcct agacctcagc cacaactcgc tgcgcgccac cgtaaaccct agcgctccga gatgcatgtg gtccagcgcc ctgaactccc tcaatctgtc gttcgctggg ctggaacagg tgcctaaagg actgccagcc aagctcagag tgctcgatct cagctgcaac agactgaaca gggcgccgca gcctgacgag ctgcccgagg tggataacct gacactggac gggaatccct tcctggtccc tggaactgcc ctcccccacg agggctcaat gaactccggc gtggtcccag cctgtgcacg ttcgaccctg tcggtggggg tgtcgggaac cctggtgctg ctccaagggg cccggggctt tgcctaagat ccaagacaga ataatgaatg gactcaaact gccttggctt caggggagtc ccgtcaggac gttgaggact tttcgaccaa ttcaaccctt tgccccacct ttattaaaat cttaaac
Protein	1 61 121 181 241 301 361	merascllll llplvhvsat tpepceldde dfrcvcnfse pqpdwseafq cvsaveveih agglnlepfl krvdadadpr qyadtvkalr vrrltvgaaq vpaqllvgal rvlaysrlke ltledlkitg tmpplpleat glalsslrlr nvswatgrsw laelqqwlkp glkvlsiaqa hspafsceqv rafpaltsld lsdnpglger glmaalcphk fpaiqnlalr ntgmetptgv caalaaagvq phsldlshns lratvnpsap rcmwssalns lnlsfagleq vpkglpaklr vldlscnrln rapqpdelpe vdnltldgnp flvpgtalph egsmnsgvvp acarstlsvg vsgtlvllqg argfa

Description of the first assignment:

Choose one human gene of interest: <CD-14>

Go to NCBI website.

Click on Entrez.

Search for CD14.

Click on gene.

Click on the first entry for CD14 antigen.

Post the sequence of the first and second exon of the gene:

Change the Graphic display to Gene Table.

Click on the first exon (coords: 1-123) and copy the sequence.

Click on the second exon (coords: 212-1456) and copy the sequence.

Post the sequence of mRNA of the gene:

Click on NM_000591 for mRNA link.

Copy the mRNA sequence at the very bottom.

Post the sequence of protein of the gene:

Click on NP_000582 for protein link.

Copy the protein sequence at the very bottom.