Toxoplasma gondii whole-cell antigens for modified agglutination test (MAT)

MAT antigen (TgMAT)

Toxoplasma gondii is an obligate intercellular parasite that infects mammals and birds. A MAT test can be used to detect anti-Toxoplasma IgG antibodies in serum, plasma and other bodily fluid samples of animals. For MAT test, formalin-treated Toxoplasma tachyzoite is used as the antigen. The presence of anti-Toxoplasma IgG antibody in the samples will cause the antigens in suspension to form a mat of cells covering the bottom of a U-shaped well. Samples without pellets at the bottom of wells are positive, samples with pellets are negative.

This whole-cell antigen was prepared using T. gondii RH strain cultivated in human foreskin fibroblast cell culture. The tachyzoites harvested from cell culture were treated with formalin, which renders tachyzoites non-viable, non-infective, and non-hazardous.

TgMAT: 1 ml, 2x10^8 tachyzoites/ml in storage buffer, can test 130 serum samples each with four titers.

MAT antigens storage condition: 4C for 6 months

Storage buffer composition: Alkaline buffer (7.02g NaCl, 3.09g boric acid (H3BO3), 24 ml of 1N NaOH, 4g of bovine plasma albumin (fraction V) in 1 liter of distilled water, adjust pH to 8.7, add sodium azide (0.1%) as a preservative), final pH 8.2-8.4. Store at 4C.

Toxoplasma gondii MAT test Kit (TgMAT Kit)

Each kit contains:
a). TgMAT antigen, 2.5 ml.
b). Alkaline Buffer, 50 ml.
c). Phosphate Buffered Saline (PBS), 50 ml.
d). Positive control serum, 60 ul.
e). Negative control serum, 60 ul.
f). Evans blue dye (2 mg/ml in H2O), 1 ml.
All reagents should be stored at 4C, except the control serum at -20C. Do not freeze TgMAT antigen!
Note: The 2.5 ml TgMAT antigens can test sixteen 96-well plates. Each plate can screen 20 serum samples. Therefore, a total of 320 samples can be screened.

The recommended protocol for MAT test is provided here.

To order MAT antigen or TgMAT Kit, visit website UT Research Foundation.


References:

Desmonts G., Remington JS. Direct agglutination test for diagnosis of Toxoplasma infection: Method for increasing sensitivity and specificity. J. Clin. Microbiol. 1980. 11:562-568.
Dubey JP, Desmonts G. Serological responses of equids fed Toxoplasma gondii oocysts. Equine Vet. J. 1987. 19:337-339.