STEVEN A. RIPP

Center for Environmental Biotechnology Tel: (865) 974-9605

676 Dabney Hall, University of Tennessee Fax: (865) 974-8086

Knoxville, TN 37996 E-mail: saripp@utk.edu

EDUCATION

Ph.D., Microbiology and Molecular Genetics, Oklahoma State University, 1996

B.S., Bacteriology, University of Wisconsin - Madison, 1989

RESEARCH INTERESTS

Construction of recombinant bacterial and bacteriophage bioreporter strains for the detection and monitoring of foodborne pathogens, biological toxicants, environmental pollutants, environmental stressor mechanisms, and as components of bio-microelectronic computer processing systems.

PROFESSIONAL EXPERIENCE

2001 – Present Research Assistant Professor, Center for Environmental Biotechnology, University of Tennessee, Knoxville

· Principle Investigator, Dynamac Corporation, “Bioluminescent monitoring of bacterial pathogens.”

· Principle Investigator, Dynamac Corporation, “Development of a phage-amplified bioreporter system for selective detection of airborne pathogens.”

· Co-Principle Investigator, USDA, “A novel whole-cell bioreporter model for foodborne toxin monitoring.”

· Co-Principle Investigator, NASA, “Bioluminescent monitoring of opportunistic pathogens in the spacecraft environment.”

· Instructor, Bacterial Genetics

1998 – Present Adjunct Faculty, Roane State Community College, Harriman, Tennessee

1998 – 2001 Senior Research Associate, Center for Environmental Biotechnology, University of Tennessee, Knoxville

· Co-Principle Investigator, NASA, “Towards intelligent distributed bioluminescence detection of biological contamination and stressors.”

1997 – 1998 Alexander Hollaender Postdoctoral Fellow, Oak Ridge National Laboratory and University of Tennessee, Knoxville

1993 – 1996 Graduate Research Assistant, Oklahoma State University, Stillwater, Oklahoma

1989 – 1993 Laboratory Manager, Loyola University Medical Center, Chicago, Illinois

HONORS AND AWARDS

Alexander Hollaender Fellowship, Oak Ridge National Laboratory, 1997-1998

Edward A. Grula Fellowship, Oklahoma State University, 1995-1996

PATENTS

Sayler, G.S., Simpson, M.L., Applegate, B.M., Ripp, S.A. “An implantable glucose sensor using a bioluminescent bioreporter integrated circuit (BBIC)”, Pending

Sayler, G.S., Ripp, S.A., Sanseverino, J. “Handheld device for the rapid detection of environmental compounds”, Pending

Sayler, G.S., Ripp, S.A., Applegate, B.A., “Detection and signal amplification of microorganisms utilizing specific bacteriophage containing luxI fusions and a bioluminescent bioreporter integrated circuit (BBIC)”, Pending

PUBLICATIONS

Ripp, Steven, David E. Nivens, and Gary S. Sayler. GEM field release investigations for bioremediation process monitoring and control. In Imanaka, T. (ed.), The Fifth International Symposium on Environmental Biotechnology, Kyoto, Japan, in press.

Ripp, Steven and Gary S. Sayler. Microbial biodegradation and bioelectronic sensing of polyaromatic hydrocarbons in the environment. In Al-Shayjij, Y. (ed.), Biotechnology Applications for the Arid Regions, Kuwait, in press.

Ripp, Steven and Gary S. Sayler. 2002. Field release of bacteria. In Bitton, G. (ed.), The Encyclopedia of Environmental Microbiology, Wiley Publishers, New York, NY.

Miller, Robert V. and Steven A. Ripp. 2001. Pseudolysogeny: A bacteriophage strategy for increasing longevity in situ, p. 79-89. In Syvanen, M. and C.I. Kado (ed.), Horizontal Gene Transfer, Academic Press, London.

Ripp, Steven, Bruce Applegate, Michael L. Simpson, and Gary S. Sayler. 2001. Whole-cell bioluminescent bioreporter sensing of foodborne toxicants, p. 13-21. In Chen, Y. R. and S. I. Tu (ed.), Photonic Detection and Intervention Technologies for Safe Food, vol. 4206. Proceedings of SPIE, Boston, MA.

Ripp, Steven, David E. Nivens, Claudia Werner, and Gary S. Sayler. Vertical transport of a field-released genetically engineered microorganism through soil. Soil Biology & Biochemistry 33:1873-1877.

Simpson, Michael L., Gary S. Sayler, G. Patterson, David E. Nivens, E. Bolton, J. Rochelle, Chris Arnott, Bruce Applegate, Steven Ripp, and M. A. Guillorn. 2001. An integrated CMOS microluminometer for low-level luminescence sensing in the bioluminescent bioreporter integrated circuit. Sensors Actuators B 72:135-141.

Cox, Chris, David E. Nivens, Steven Ripp, Meng Meng Wong, Anthony Palumbo, Robert S. Burlage, and Gary S. Sayler. 2000. An intermediate-scale lysimeter facility for subsurface bioremediation research. Bioremediation Journal 4:69-79.

Hay, Anthony, Steven Ripp, and Gary S. Sayler. 2000. Catabolic plasmids. In Lederberg, L. (ed.), The Encyclopedia of Microbiology, 2nd edition, Academic Press, San Diego, CA, p. 730-744.

Ripp, Steven, Bruce M. Applegate, David E. Nivens, Michael J. Paulus, George E. Jellison, Michael L. Simpson, and Gary S. Sayler. 2000. Whole-cell environmental monitoring devices: bioluminescent bioreporter integrated circuits (BBICs). In Mulchandani, A., and O.A. Sadik (ed.), Recent Advances in Environmental Chemical Sensors and Biosensors, ACS Press, Clarendon Hills, IL, p. 197-205.

Ripp, Steven, David E. Nivens, Yeonghee Ahn, Claudia Werner, John Jarrell, James Easter, Robert Burlage, and Gary S. Sayler. 2000. Controlled field release of a bioluminescent genetically engineered microorganism for bioremediation process monitoring and control. Environmental Science and Technology 34:846-853.

Ripp, Steven, Claudia Werner, David E. Nivens, and Gary S. Sayler. 2000. Most-probable-number enumeration of lux-based bioluminescent microorganisms. Applied Microbiology and Biotechnology 53:736-741.

Sayler, Gary S. and Steven Ripp. Field applications of genetically modified bacteria for bioremediation processes. 2000. Current Opinion in Biotechnology 11:286-289.

Ripp, Steven, David E. Nivens, Robert Burlage, and Gary S. Sayler. 1999. Field-scale bioremediation monitoring utilizing bioluminescent genetically engineered microorganisms. In Leeson, A. and B.C. Alleman (ed.), Bioremediation Technology for Polycyclic Aromatic Hydrocarbon Compounds, Battelle Press, Columbus, OH, p. 277-282.

Applegate, Bruce, Justin Shingleton, Steven Ripp, Nathan Bright, David Nivens, Michael Simpson, and Gary Sayler. 1998. Bioluminescent bioreporter integrated circuits (BBICs) for the detection of toluene and trichloroethylene (TCE) in aqueous solutions. Proceedings of the International Society for Bioluminescence and Chemiluminescence, Bologna, Italy.

Miller, Robert, V. and Steven Ripp. 1998. The importance of pseudolysogeny to transduction in situ. In Syvane, M. and C. Kado (ed.), Horizontal Gene Transfer, Chapman and Hall, London.

Ripp, Steven, and Robert V. Miller. 1998. Dynamics of the pseudolysogenic response in slowly growing cells of Pseudomonas aeruginosa. Microbiology 144:2225-2232.

Sayler, Gary S., Chris D. Cox, Robert Burlage, Steven Ripp, David E. Nivens, Claudia Werner, Yeonghee Ahn, and Udayakumar Matrabuthum. 1998. Field application of a genetically engineered microorganism for polycyclic aromatic hydrocarbon bioremediation process monitoring and control. In Fass, R., Y. Flashner, and S. Reuveny (ed.), Novel Approaches for Bioremediation of Organic Pollution, 42nd OHOLO Conference, Eilat, Israel.

Simpson, Michael, Michael Paulus, Gerald Jellison, Gary Sayler, Bruce Applegate, Steven Ripp, and David Nivens. 1998. Bioluminescent bioreporter integrated circuits (BBICs): whole-cell chemical biosensors. Technical Digest of the 1998 Solid-State Sensors and Actuators Conference, Hilton Head, S.C., p. 354-357.

Simpson, Michael L., Gary S. Sayler, Steven Ripp, David E. Nivens, Bruce M. Applegate, Michael J. Paulus, and Gerald E. Jellison Jr. 1998. Bioluminescent bioreporter integrated circuits form novel whole-cell biosensors. Trends in Biotechnology 16:332-338.