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OVERVIEW

There are several different procedures for sequencing a segment of DNA.

Dideoxy sequencing  relies on copying a single fragment of DNA many times but each copy is prematurely terminated.  This produces a series of nested DNA copies, each differing by a single base in length. These fragments are separated on a polyacrylamide gel and the DNA sequence is determined by reading the the terminating base of each fragment on the gel.

This process is presented in diagrams and photographs.   Slides 2-14 diagram the process of creating nested fragments.  Slides 15 - 25 are photographs of the preparation  of a polyacrylamide gel for separation of these fragments and results obtained after electrophoresis.

Photographs were taken at the Automated Sequencing Facility at the University of Tennessee with the help of Neil Quigley, Director of the facility.